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目的 研究点燃形成过程中和点燃后大鼠海马中谷氨酸天门冬氨酸转运体 (GL AST)和谷氨酸转运体 1(GL T- 1)的变化 ,进一步探讨癫痫的形成机制。方法 将 78只雄性成年 Wistar大鼠随机分为对照组( 组 )和戊四氮 (PTZ)组 ( 组 )。 组腹腔注射阈下剂量的 PTZ(35 mg/ kg) ,每日 1次 ,直至达到点燃标准 ; 组腹腔注射等量生理盐水。采用 RT- PCR方法检测海马区 GL AST和 GL T- 1m RNA的表达。结果 PTZ组点燃后 ,GL AST m RNA的表达下降 ,6 0天时恢复至对照组水平 ;与对照组比较 ,PTZ组 GL T- 1m RNA的表达 ,在给药后 15天时开始上升 ,点燃后 0小时和 48小时显著升高 ,此后呈下降趋势。 6 0天时 ,两组比较无明显差异。结论 海马区胶质细胞谷氨酸转运体的下降可能与癫痫敏感性的形成有关。
Objective To investigate the changes of glutamate aspartate transporter (GL-AST) and glutamate transporter 1 (GL-1) in the hippocampus of rats during and after ignition to further explore the mechanism of epilepsy. Methods Seventy-eight male adult Wistar rats were randomly divided into control group (group) and PTZ group (group). Group intraperitoneal injection of subthreshold dose of PTZ (35 mg / kg), once daily until the ignition standard; group intraperitoneal injection of normal saline. RT-PCR was used to detect the expression of GL-AST and GL-1mRNA in hippocampus. Results The expression of GL-AST m RNA in PTZ group was decreased and returned to control level on the 60th day. Compared with the control group, the expression of GL-1mRNA in PTZ group started to increase 15 days after the administration, Hour and 48 hours significantly increased, then a downward trend. 60 days, no significant difference between the two groups. Conclusions The decrease of glial cell glutamate transporter in hippocampus may be related to the formation of epilepsy sensitivity.