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目的探讨内质网分子伴侣家族成员Grp94、Grp78和PDI在幽门螺杆菌感染者胃黏膜组织中的表达及意义。方法应用半定量RT-PCR方法及Westernblot方法分别检测感染与未感染幽门螺杆菌的人胃黏膜组织中Grp94、Grp78和PDI的mRNA及蛋白的表达情况。结果未感染组28例,Grp94、Grp78和PDI的mRNA表达量分别为(0.427±0.0360)、(0.8038±0.500)和(0.5198±0.0379),感染组32例,Grp94、Grp78和PDImRNA表达量为(0.882±0.082)、(1.1586±0.0839)和(1.0642±0.1533)。两组相比,感染组的Grp94、Grp78和PDImRNA的表达量明显高于未感染组(P<0.01);未感染组Grp94、Grp78和PDI蛋白表达量分别为(0.427±0.0360)、(0.4345±0.0545)和(0.5198±0.0379),感染组Grp94、Grp78和PDI蛋白表达量为(0.671±0.072)、(0.6204±0.0412)和(0.8252±0.032)。两组相比,感染组Grp94蛋白的表达量明显高于未感染组(P<0.01)。结论幽门螺杆菌感染可使人胃黏膜增加Grp94、Grp78和PDImRNA的表达及蛋白的合成,这可能有利于胃黏膜的自身保护作用。
Objective To investigate the expression and significance of Grp94, Grp78 and PDI in the molecular chaperons of endoplasmic reticulum in gastric mucosa of Helicobacter pylori-infected patients. Methods The mRNA and protein expressions of Grp94, Grp78 and PDI in gastric mucosa of infected and non-infected H. pylori were detected by semi-quantitative RT-PCR and Western blot respectively. Results The mRNA expression of Grp94, Grp78 and PDI in uninfected group was (0.427 ± 0.0360), (0.8038 ± 0.500) and (0.5198 ± 0.0379), respectively. In the infected group, the mRNA expression of Grp94, 0.882 ± 0.082), (1.1586 ± 0.0839) and (1.0642 ± 0.1533), respectively. The expression of Grp94, Grp78 and PDImRNA in infected group was significantly higher than that in non-infected group (P <0.01). The expression of Grp94, Grp78 and PDI protein in untreated group were (0.427 ± 0.0360) and (0.4345 ± The expression of Grp94, Grp78 and PDI in infected group were (0.671 ± 0.072), (0.6204 ± 0.0412) and (0.8252 ± 0.032), respectively. The expression of Grp94 protein in infected group was significantly higher than that in non-infected group (P <0.01). Conclusion Helicobacter pylori infection can increase the expression of Grp94, Grp78 and PDImRNA and the synthesis of protein in gastric mucosa, which may be beneficial to the protective effect of gastric mucosa.