应用PCR方法对致病性外瓶霉进行分类鉴定。以ITS3和ITS4为引物 ,对常见的7种致病性外瓶霉的模式株核糖体DNA转录间隔区(ITSⅡ)进行扩增 ,4种内切酶(HinFI、MspI、BsuRI和RsaI)酶切。各种间多态性显著 ,常规方法难以鉴别的皮炎外瓶霉和甄氏外瓶霉较易区分。PCR RFLP准确可靠 ,可用于形态及其他方法难以确定的致病菌种的鉴别。 The PCR method was used to classify and identify the pathogenic Ectomycorrhizal fungi. ITS3 and ITS4 were used as primers to amplify the ribosomal DNA transcribed spacer (ITS Ⅱ) of seven strains of endophytic E. epidermidis. Four endonucleases (HinFI, MspI, BsuRI and RsaI) . All kinds of polymorphism was significant, the conventional method is difficult to identify Dermatitis exocarp and Chen outside Epidermidis easier to distinguish. PCR RFLP is accurate and reliable, which can be used for identification of pathogenic species difficult to determine by morphology and other methods.