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目的了解北京市门头沟区食品被食源性致病菌的污染状况,确定食源性致病菌种类,为食源性疾病的预防控制提供科学依据。方法依据《北京市食源性致病菌监测工作手册》和《食品安全国家标准食品微生物学检验》GB 4789进行检测。结果 2010—2012年共采集食品样本18种351件,进行沙门菌、单核细胞增生李斯特菌、金黄色葡萄球菌、肠出血性大肠埃希菌O157:H7、副溶血性弧菌、志贺菌、蜡样芽孢杆菌、阪崎肠杆菌数、创伤弧菌等项目检测。共检出食源性致病菌24株,总检出率为6.84%。其中副溶血性弧菌检出率为22.22%(12/54);其次为阪崎肠杆菌检出率为10.00%(2/20),创伤弧菌检出率为5.88%(2/34),单核细胞增生李斯特菌检出率为1.52%(4/264),金黄色葡萄球菌检出率为1.50%(4/267),沙门菌、肠出血性大肠埃希菌O157:H7、志贺菌、蜡样芽孢杆菌、大肠埃希氏菌均未检出。生食动物性水产品污染率为25.93%(14/54),其次为婴幼儿食品污染率为20.00%(2/10)。结论门头沟区市售食品中存在食源性致病菌污染,主要在生食动物性水产品和婴幼儿食品中检出。生食动物性水产品被副溶血性弧菌污染,婴幼儿食品被阪崎肠杆菌污染。
Objective To understand the food contamination status of food-borne pathogenic bacteria in Mentougou District of Beijing and determine the types of food-borne pathogenic bacteria to provide a scientific basis for the prevention and control of food-borne diseases. Methods According to “Beijing Foodborne Pathogen Monitoring Manual” and “National Food Safety Microbiological Test” GB 4789 for testing. Results A total of 351 food samples were collected from 2010 to 2012 for Salmonella, Listeria monocytogenes, Staphylococcus aureus, Enterohemorrhagic Escherichia coli O157: H7, Vibrio parahaemolyticus, Shiga Bacillus cereus, Bacillus cereus, Enterobacter sakazakii, Vibrio vulnificus test. A total of 24 foodborne pathogens were detected, with a total detection rate of 6.84%. The detection rate of Vibrio parahaemolyticus was 22.22% (12/54), followed by Enterobacter sakazakii (10.00%) and Vibrio vulnificus (5.88%, 2/34) , Listeria monocytogenes detection rate was 1.52% (4/264), Staphylococcus aureus detection rate was 1.50% (4/267), Salmonella, enterohemorrhagic Escherichia coli O157: H7, Shigella, Bacillus cereus, Escherichia coli were not detected. The contamination rate of raw animal aquatic products was 25.93% (14/54), followed by infant food contamination rate of 20.00% (2/10). Conclusion Food-borne pathogens are found in commercially available food in Mentougou District, which are mainly detected in raw animal-derived aquatic products and infant food. Raw food animal aquatic products are contaminated by Vibrio parahaemolyticus, infant food contaminated with E. sakazakii.