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目的本实验旨在观察脑缺血再灌损伤中是否有补体参与，其参与是否与组织坏死有关，蛇毒制剂降纤酶对其有无干预作用。方法制成大鼠大脑中动脉缺血2h再灌22h动物模型，运用免疫组化方法检测再灌组织中的补体沉积，并给予降纤酶处理，观察其干预效果。结果补体C3在缺血再灌组织中大量沉积，为强阳性；降纤酶干预后补体沉积减少，为弱阳性；正常组及假手术组脑组织中无补体沉积。经X2检验证实补体沉积与组织坏死关系密切（P＜0．01）。给药组白细胞浸润较未给药组明显减少（P＜0．05），组织损伤程度也相对较轻。结论脑缺血再灌组织中有大量补体参与，补体的参与同组织坏死有密切关系。蛇毒制剂降纤酶可减少缺血再灌组织中补体的沉积，从而削减白细胞浸润，减轻脑组织损伤程度。
Objective The purpose of this experiment is to observe whether there is complement involvement in cerebral ischemia-reperfusion injury, whether its involvement is related to tissue necrosis or not, and whether defibrase of snake venom preparation has intervention effect on it. Methods The rat model of middle cerebral artery ischemia 2h and reperfusion 22h was made. The complement deposition in the reperfusion tissue was detected by immunohistochemistry, and the defibrase was treated to observe its intervention effect. Results Complement C3 was strongly positive in ischemic reperfusion tissue. Complement deposition decreased after defibrase treatment, which was weakly positive. No complement deposition was observed in normal and sham-operated brain tissue. The X2 test confirmed that complement deposition and tissue necrosis were closely related (P <0.01). Compared with the untreated group, the infiltration of leukocytes in the administration group was significantly reduced (P <0.05), and the degree of tissue damage was also relatively light. Conclusion There is a large number of complement involved in cerebral ischemia-reperfusion and the involvement of complement is closely related to tissue necrosis. The snake venom preparation defibrase can reduce the deposition of complement in ischemia-reperfusion tissue, thus reducing leukocyte infiltration and lessening the damage of brain tissue.