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用引物PL1－PL2PCR扩增对虾病原菌——坎普氏弧菌（V．campbellii）2－5B菌株16SrRNA基因-1223bP的片段，采用pUC19质粒构建dT载体法完成该片段的克隆。部分序列测定及分析结果表明，该菌株与GenBank中坎普氏弧菌标准株序列之间同源性为96.94％。所测序列可为PCR特异性引物及寡核苷酸探针设计提供依据，进而应用于病原菌的检测和快速鉴定。
A fragment of 16S rRNA gene-1223bP from the shrimp pathogen, V. cambbellii 2-5B, was amplified by using the primer PL1-PL2PCR, and cloned using the pUC19 plasmid to construct the dT vector. Partial sequence analysis and analysis showed that the homology between this strain and the standard strain of Campylobacter in GenBank was 96.94%. The tested sequences can provide the basis for the design of PCR-specific primers and oligonucleotide probes, and can be applied to the detection and rapid identification of pathogenic bacteria.