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目的:观察大鼠腹部开放伤合并海水浸泡后不同时段血浆内毒素(LPS)、肿瘤坏死因子-α(TNF-α)、IL-6和IL-8的变化。方法:成年健康Wistar大鼠116只,随机分为2组,其中腹部开放伤合并海水浸泡组96只,腹部开放伤对照组20只。应用微生物快速检测仪和γ测量仪检测大鼠腹部开放伤合并海水浸泡后0.5,1,2,3和4h血浆LPS、TNF-α、IL-6和IL-8水平。结果:腹部开放伤合并海水浸泡2～4h后,血浆中LPS水平明显上升,与对照组比较具有显著性差异。海水浸泡4h后血浆中TNF-α浓度上升明显,具有显著性差异。海水浸泡3～4h后血浆中IL-6浓度明显升高,具有显著性差异。海水浸泡后不同时间血浆中IL-8浓度与对照组比较无明显变化。结论:血浆中LPS、TNF-α和IL-6浓度明显升高与损伤关系密切,是引发中毒性休克导致机体死亡的主要原因之一。
Objective: To observe the changes of plasma lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α), interleukin-6 and interleukin-8 in open abdominal wound and seawater immersion in rats. Methods: One hundred and sixty healthy adult Wistar rats were randomly divided into two groups, including 96 cases of abdominal open injury combined with seawater immersion group and 20 cases of abdominal open injury control group. The plasma LPS, TNF-α, IL-6 and IL-8 levels were measured at 0.5, 1, 2, 3 and 4 h after open abdomen injury and seawater immersion in rats by using the rapid microbial test and γ measuring instrument. Results: The level of LPS in plasma increased significantly after 2 ~ 4 hours of open abdominal injury and seawater immersion, which was significantly different from the control group. Plasma concentrations of TNF-α increased significantly after 4 h of seawater immersion, with significant differences. After immersion in sea water for 3 ~ 4h, the concentration of IL-6 in plasma was significantly increased, with significant difference. The concentration of IL-8 in plasma at different times after seawater immersion had no significant change compared with the control group. Conclusion: Plasma LPS, TNF-α and IL-6 levels are significantly increased and are closely related to injury, which is one of the main causes of death caused by toxic shock.