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尿沉渣的细胞学检查,是诊断肾脏疾病与观察病情经过的一个重要方法。但光镜下的观察均为单项报道。为了寻求在一份标本中可检测有核细胞与红细胞成分的变化结果,现将我院近年采用的染色方法报告如下。材料来源:为本院儿科病房诊断为肾脏病的30名患儿,其中急性肾炎15名,肾炎性肾病7名,单纯性肾病8名,于午睡后每人取新鲜尿液10ml进行检查。同时以本院托儿所30名健康儿童的新鲜尿液,每人10ml做对照检查。方法:取排尿后30分钟内的新鲜尿液10ml,以每分钟2000转进行离心,取沉渣液1滴,滴于含一小滴新鲜人血清的玻璃片上涂膜,待自然干燥后,用瑞氏
Urinary sediment cytology, is to diagnose kidney disease and observe the condition is an important method. However, the observations under light microscope are reported individually. In order to seek in a specimen can detect changes in nucleated cells and erythrocyte composition results, now in our hospital using the staining method reported in recent years are as follows. MATERIALS: 30 children diagnosed as kidney disease in our pediatric ward, including 15 with acute nephritis, 7 with nephritic nephropathy and 8 with simple nephropathy, were examined with 10ml of fresh urine after taking a nap. At the same time to our nursery 30 healthy children’s fresh urine, 10ml per person to do the control check. Methods: Take 10ml of fresh urine within 30 minutes after urination and centrifuge at 2000 revolutions per minute. Take 1 drop of sediment solution and apply it to glass slides containing a droplet of fresh human serum. After drying naturally, ’S