植株的操作技术 (一)培养前的准备在着手进行花粉培养之前,应准备好所需药品、用具及玻璃器皿,做好玻璃器皿、培养用的试管或三角烧瓶的清洗等。工作初开展时,每一步操作都应按严格要求进行。 (二)培养基的配制要根据不同的作物的不同要求,选择好培养基。基本培养基的成分按其性质和含量可以分成大量元素无机盐,微量元素无机盐,有机生长物质,蔗糖,洋菜和水几部分,做试验时再加入附加成分。在配制培养基时,先把各部分配成培养基 Plant operation techniques (A) Preparation before training Before proceeding with the pollen culture, should be prepared for the required drugs, utensils and glassware, glassware, culture tubes or conical flask cleaning and so on. At the beginning of the work, every step should be carried out according to strict requirements. (B) the preparation of medium According to the different requirements of different crops, choose a good medium. Basic medium composition according to their nature and content can be divided into a large number of elements of inorganic salts, trace inorganic salts, organic growth substances, sucrose, agar and water several parts, add additional ingredients to do the test. In the preparation of medium, the first part of the medium dubbed