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目的:建立HPLC法测定阿归养血颗粒中阿魏酸含量的方法。方法:采用HYPERSIL OSD2 C_(18)色谱柱(150mm×4.6 mm,5μm),乙腈-1%冰醋酸(30:70)为流动相;流速1.0ml·min~(-1);检测波长320nm。结果:阿魏酸在2.2～15.4μg·min~(-1)范围内线性关系良好(r=0.999 9),平均回收率为99.7%,RSD=0.9%(n=5)。结论:方法简便,专属性强,结果准确,可用于阿归养血颗粒的质量控制。
Objective: To establish a HPLC method for determination of ferulic acid in Aguiyang Yangxue Granules. METHODS: A Hypersil OSD2 C18 column (150 mm×4.6 mm, 5 μm) was used with acetonitrile-1% glacial acetic acid (30:70) as the mobile phase. The flow rate was 1.0 ml·min-1 and the detection wavelength was 320 nm. Results: The ferulic acid had good linearity in the range of 2.2～15.4μg·min -1 (r=0.999 9). The average recovery was 99.7%, RSD=0.9% (n=5). Conclusion: The method is simple, specific and accurate. It can be used for the quality control of Aguiyang Yangxue granules.