论文部分内容阅读
目的探讨不同剂量的血管生成抑制素(Angiostatin)在胶质瘤治疗中的抑制血管生成作用。方法应用不同剂量(100ng、1000ng)的血管生成抑制素分别作用于体外培养的大鼠C6脑胶质瘤细胞系和C6/SD大鼠脑胶质瘤模型,分别计算细胞存活率和抑瘤生成率,SABC免疫组织化学技术检测体内胶质瘤中的微血管密度。结果不同剂量的血管生成抑制素均对体外培养的胶质瘤细胞的生长不产生明显抑制作用;对体内生长的胶质瘤则有显著抑制作用,而且较高剂量的Angiostatin的抑制作用更为明显,抑瘤率最高达65.3%(P<0.01)。经不同剂量的血管生成抑制素处理的脑胶质瘤中微血管密度较对照组降低(P<0.01)。结论血管生成抑制素能抑制C6脑胶质瘤的血管生成,且较高剂量实验组的作用更为明显,此研究对血管生成抑制素抑制C6胶质瘤的生长机制研究奠定了一定基础。
Objective To investigate the anti-angiogenic effects of different doses of Angiostatin in the treatment of glioma. Methods Angiostatin with different dosages (100ng, 1000ng) were used in C6 rat glioma cell line and C6 / SD rat glioma model respectively. The cell viability and anti-tumor growth were calculated Rate, SABC immunohistochemical detection of microvessel density in vivo glioma. Results Different doses of angiostatin had no significant inhibitory effect on the growth of glioma cells cultured in vitro, significant inhibitory effect on the growth of glioma in vivo, and the inhibitory effect of higher doses of Angiostatin was more obvious , The tumor inhibition rate was up to 65.3% (P <0.01). Microvessel density in gliomas treated with different doses of angiostatin was lower than that in control group (P <0.01). Conclusion Angiostatin can inhibit the angiogenesis of C6 glioma, and the effect of higher dose of the experimental group is more obvious. This study lays a foundation for the study of the inhibitory effect of angiostatin on the growth of C6 glioma.