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Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little is known about the role of ficolin-2 in human immunodeficiency virus(HIV) infection. Here, we describe our novel findings that serum ficolin-2concentrations of 103 HIV-1 patients were much higher compared to those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV-1 envelope glycoprotein gp120, and subsequently induce complement dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells(TZM-b1 and MT-2 cells) and infection in a ficolin-2 dosedependent manner. To our knowledge, this is the first report about the protective role of ficolin-2against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.
Ficolin-2 is a lectin complement pathway activator present in normal human plasma and usually associated with infectious diseases, but little known to the role of ficolin-2 in human immunodeficiency virus (HIV) infection. Here, we describe our novel findings that serum Ficolin-2 concentrations of 103 HIV-1 patients were much higher than those of 57 healthy donors. In vitro analysis showed that HIV-1 infection could enhance ficolin-2 expression. We further demonstrated that recombinant ficolin-2 protein could bind with HIV- 1 envelope glycoprotein gp120, and either prompted to contribute dependent cytotoxicity. Moreover, ficolin-2 could block the entry of HIV-1 into target cells (TZM-b1 and MT-2 cells) and infection in a ficolin-2 dose dependent manner. knowledge, this is the first report about the protective role of ficolin-2against HIV-1 infection and our study suggests that ficolin-2 is an important human innate immune molecule against HIV.