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以对霜霉病不同抗性的葡萄品种左优红和霞多丽为材料,利用分子生物学和植物生理学试验手段,结合药理学试验,探讨葡萄在应答霜霉病过程中葡萄多磷酸肌醇激酶基因(VvIPK2)和H2O2的作用机制。接种霜霉病菌后15 h葡萄叶片VvIPK2表达量是正常水平的12倍,接种后3 hH2O2含量达最大值,同时苯丙氨酸解氨酶和几丁质酶活性升高;多磷酸肌醇激酶(IPK2)抑制剂、外源H2O2及H2O2清除剂均能改变霜霉病菌所引起的抗性葡萄品种左优红叶片PAL和几丁质酶活性的变化,同时可以影响不同抗性品种叶片的感病情况;IPK2抑制剂对葡萄霜霉病菌引起的H2O2水平变化没有影响;清除H2O2可减弱葡萄霜霉病菌对VvIPK2表达量的诱导效应。研究表明H2O2位于IPK2的上游,通过调控PAL和几丁质酶活性参与葡萄应答霜霉病过程。
Taking the grape varieties Zuyouhong and Chardonnay, which have different resistance to downy mildew, as materials, molecular phytoextraction and plant physiology test were used in combination with pharmacological tests to investigate the effect of grape polypis inositol Mechanism of action of kinase gene (VvIPK2) and H2O2. The expression of VvIPK2 in grapevine leaves was 12 times higher than the normal level after inoculation with downy mildew, and the content of H 2 O 2 reached the maximum at 3 h after inoculation. At the same time, the activity of phenylalanine ammonia lyase and chitinase increased. Inositol polyphosphate kinase (IPK2) inhibitors, exogenous H2O2 and H2O2 scavengers could change the changes of PAL and chitinase activities in resistant leaves of resistant grape cultivars caused by downy mildew, and at the same time, they could affect the leaves of different resistant varieties The effect of IPK2 inhibitor on the level of H2O2 induced by Plasmopara viticola was not affected. H2O2 scavenging could reduce the induction effect of Plasmopara viticola on VvIPK2 expression. Studies have shown that H2O2 is located upstream of IPK2 and participates in grape downy mildew process by regulating PAL and chitinase activities.