目前,应用地高辛标记探针检测Vero细胞残余DNA含量仍是最常用的检测方法,该方法具有特异性强、无放射污染、操作简便等特点。在实际操作中,地高辛探针的灵敏度及杂交的条件对检测结果均有很大的影响。为此,我们就提高探针灵敏度及优化杂交条件进行了摸索。 At present, it is still the most commonly used method to detect residual DNA content in Vero cells by using digoxigenin-labeled probe. The method is characterized by strong specificity, no radioactive contamination and simple operation. In practice, the sensitivity of the digoxigenin probe and the conditions of hybridization have a great impact on the test results. To this end, we have explored ways to increase probe sensitivity and optimize hybridization conditions.