Objective: This study aimed to investigate previously observed side effects, i .e. increased epileptic activity during clonidine medication. The safety and eff ectiveness of clonidineas spike inducing agent compared to sleep deprivation wer e tested. Methods: Patients suffering from drug-resistant localization related epilepsy took part in 3 magnetoencephalography(MEG) sessions. One session was re corded without any activating measures. The other two sessions were either perfo rmed after sleep deprivation or after medication with clonidine. Target paramete r was the number of spikes or sharp-waves during a 30 min recording period. Res ults: About 67%of the patients showed increased spike activity after clonidine, whereas sleep deprivation increased the number of spikes in 33%, and 29%of the patients did not show any activatio n at all. Clonidine was most effective in temporal lobe epilepsy, when the focus was located in the right hemisphere, and when clonidine serum concentrations we re ranging between 0.6 and 1.0 ng/ml. Conclusions:This study showed for the firs t time that clonidine can be considered an effective spike or sharp-wave induci ng drug that is superior to the potency of sleep deprivation. Significance: The administration of clonidine increases the probability of recording ictal and int erictal epileptic activity during limited acquisition time. Objective: This study aimed to previously observed side effects, i. E. Increased epileptic activity during clonidine medication. The safety and effctiveness of clonidineas spike inducing agent compared to sleep deprivation wer e tested. Methods: Patients suffering from drug-resistant localization One session was re-corded without any activating measures. The other two sessions were either perfo rmed after sleep deprivation or after with clonidine. Target paramete r was the number of spikes or sharp- waves during a 30 min recording period. Res ults: About 67% of the patients showed increased spike activity after clonidine, compared to sleep deprivation increased the number of spikes in 33%, and 29% of the patients did not show any activatio n at all . Clonidine was most effective in temporal lobe epilepsy, when the focus was located in the right hemisphere, and when clonidine serum concentrations we e ranging between 0.6 and 1.0 ng / ml. Conclusions: This study showed for the firs t time that clonidine can be considered an effective spike or sharp-wave induci ng drug that is superior to the potency of sleep deprivation. Significance: The administration of clonidine increases the probability of recording ictal and int erictal epileptic activity during limited acquisition time.