论文部分内容阅读
利用已经连续培养 35～ 38代、其腋芽增殖生长能力已衰退的香椿试管苗为材料 ,研究了Thidiazuron(TDZ)对叶、叶柄、茎段外植体形成愈伤组织的反应以及对腋芽增殖生长、生根的影响 ,结果显示 ,TDZ+0 .2 m g/ L NAA能使不同外植体形成愈伤组织的能力显著提高 ,其中茎段形成愈伤组织的能力较强。 TDZ小于0 .0 2 mg/ L时对芽的增殖生长起促进作用 ,而且可恢复腋芽旺盛的分枝增殖能力 ;TDZ高于 0 .0 2 mg/ L 则全面抑制生长 ,并随质量浓度的升高变态苗增多 ;经 TDZ培养过的试管苗其生根率并未降低。
In vitro culture of Toona sinensis seedlings, which had been cultivated for 35-38 passages in vitro, was used to study the effects of Thidiazuron (TDZ) on the callus formation of explants in leaves, petioles and stems, The results showed that TDZ + 0.2 mg / L NAA could significantly improve the ability of callus formation of different explants, and the ability of stem segments to form callus was stronger. When TDZ is less than 0.02 mg / L, the proliferation and growth of shoots are promoted, and the branch proliferation ability of axillary buds is restored. TDZ higher than 0.026 mg / L inhibits the growth of buds completely and decreases with the increase of mass concentration Increased metamorphosis increased; TDZ cultured plantlets did not reduce its rooting rate.