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PBMNC经CD~3McAb作用培养诱导LAK细胞,国内未见有报道。我们先计数患者PBMNC数,根据PBMNC数的多少决定取血量,一般取血5～10ml可分离到2～4.5×10~7个PBMNC。PBMNC经CD~3McAb和rIL-2联合培养诱导LAK细胞96小时,细胞扩增2.1～3.4倍,经24份临床治疗标本培养LAK细胞的单向观察和4份正常人标本培养LAK细胞的平行对照观察,均显示以96小时培养的LAK细胞活性最强。平均杀瘤活性,CD~3McAb~+96小时比144小时高1.3倍(P<0.01),CD~3McAb~+96小时比144小时高1.1倍(P<0.02)。说明培养LAK细胞存在时间曲线,一般不宜超过一周。平行对照观察显示,加入CD~3McAb培养的LAK细胞,无论是扩增数量还是杀瘤活性均优于
PBMNC induced by CD ~ 3McAb induced LAK cells, no domestic reports. We first count the number of patients with PBMNC, according to the number of PBMNC decided to take the amount of blood, the general blood 5 ~ 10ml can be separated to 2 ~ 4.5 × 10 ~ 7 PBMNC. LAK cells were induced by CD ~ 3McAb and rIL-2 for 96 hours and proliferated by 2.1-3.4 times. After one-way observation of LAK cells cultured in 24 clinical specimens and parallel control of four normal human LAK cells Observations showed that LAK cells cultured at 96 hours had the strongest activity. The average oncolytic activity was 1.3 times (P <0.01) higher than that of 144 hours CD ~ 3McAb ~ + 96 hours and 1.1 times higher than that of 144 hours CD ~ 3McAb ~ + 96 hours (P <0.02). Explain the existence of LAK cell culture time curve, generally not more than a week. The parallel control observation showed that LAK cells cultured in CD ~ 3McAb were better than the number of amplification or tumor killing activity