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从细胞周期调控这一途径探讨视黄酸抑制细胞增殖、诱导细胞分化的分子机制。结果显示：５×１０－６ｍｏｌ／Ｌ全反式视黄酸（ＡＴＲＡ）及芳维甲（ＡＥ）处理的ＨＬ－６０细胞生长受抑，４ｄ后９０％细胞停滞于Ｇ０／Ｇ１期，Ｇ１→Ｓ期转换受阻，同时ＮＢＴ还原反应增强，ＷｅｓｔｅｒｎＢｌｏｔ分析及免疫细胞化学染色表明处理后的细胞ｐ１６、ｐ２１两种细胞周期素依赖激酶抑制物（ＣＫＩｓ）表达增加，而ＣｙｃｌｉｎＤ１和ｐＲｂ蛋白则降低，其中尤以ＡＴＲＡ作用为明显。结果提示，细胞周期调控相关蛋白可能在视黄酸及其衍生物抑制细胞增殖、诱导细胞分化过程中起重要作用。
From the cell cycle regulation pathway, the molecular mechanism of retinoic acid inhibiting cell proliferation and inducing cell differentiation was explored. The results showed that the growth of HL-60 cells treated with 5×10 -6 mol/L all-trans-retinoic acid (ATRA) and arylvinylpyrrolidone (AE) was inhibited, and 90% of the cells were arrested in G0/G1 phase after 4 days, G1→ S phase transition was blocked, and NBT reduction was enhanced. Western Blot analysis and immunocytochemistry showed that the expression of two cyclin-dependent kinase inhibitors (CKIs) in p16 and p21 cells was increased, while CyclinD1 and pRb proteins were decreased. In particular, the effect of ATRA is obvious. The results suggest that cell cycle regulation-related proteins may play an important role in the inhibition of cell proliferation and induction of cell differentiation by retinoic acid and its derivatives.