采用 MTT比色、荧光技术和放免技术观察了猪肺动脉低氧内皮细胞条件培养液 (HECCM)和 1- (2 ,6 -二甲基苯氧基 ) - 2 - (3,4-二甲氧基苯乙胺基 )丙烷盐酸盐 (DDPH)对猪肺动脉平滑肌细胞 (PASMCs)增殖、第二信使 ([Ca2 +]i 和 c AMP)的影响。结果显示 :1HECCM促进 PASMC增殖 ,而 DDPH可抑制之 ,抑制率为 77.46 %。 2HECCM升高 PASMCs内 [Ca2 +]i,而 DDPH可对抗此作用。 3HECCM组 PASMCs内 c AMP含量明显低于对照组(NECCM组 ) ,而 HECCM+DDPH组 PASMCs内 c AMP与 NECCM组无显著性差异 The effects of HECCM and 1- (2,6-dimethylphenoxy) -2 - (3,4-dimethoxy-2-methoxyphenoxy) Phenylethylamine) propane hydrochloride (DDPH) on the proliferation of porcine pulmonary artery smooth muscle cells (PASMCs), the second messenger ([Ca2 +] i and c AMP). The results showed that 1HECCM could promote the proliferation of PASMC, while DDPH could inhibit it with the inhibition rate of 77.46%. 2HECCM increased [Ca2 +] i in PASMCs, whereas DDPH counteracted this effect. The level of cAMP in PASMCs in 3HECCM group was significantly lower than that in control group (NECCM group), but there was no significant difference between HECCM + DDPH group and NECCM group