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目的复制尾静脉注射和腹腔注射接种的裸鼠乳腺癌转移瘤模型,应用生物发光技术监测肿瘤细胞在活体内的生长和转移。方法培养转染有荧光素酶基因的人乳腺癌细胞系MDA-MB-231-luc-D3-h2LN,分别通过尾静脉和腹腔接种到裸鼠体内,每周进行生物发光成像,最后解剖裸鼠体外观察转移灶。结果尾静脉接种模型肿瘤细胞在体内1周时有明显减少,之后在肺部定植并迅速增长,于接种后5周体外成像,确认肺部转移,其他部位未见肿瘤转移灶;腹腔接种模型的肿瘤细胞在腹腔形成多个转移灶,肿瘤细胞持续增殖,于接种后6周体外成像,发现壁层腹膜和脏层腹膜内多个转移灶,各脏器和腹腔以外部位未见肿瘤转移。结论建立了两种裸鼠转移瘤模型,并应用生物发光成像技术实现了深层部位肿瘤的追踪监测。
Objective To observe the growth and metastasis of tumor cells in vivo by using bioluminescence technology to replicate the model of metastatic breast cancer in nude mice by tail vein injection and intraperitoneal injection. Methods The human breast cancer cell line MDA-MB-231-luc-D3-h2LN transfected with luciferase gene was cultured and inoculated into the nude mice respectively through the tail vein and peritoneal cavity. Bioluminescence imaging was performed weekly. At last, nude mice Metastasis in vitro observation. Results The tail vein inoculation model significantly reduced the number of tumor cells at 1 week in vivo, followed by colonization and rapid growth in the lungs. Imaging was performed 5 weeks after inoculation to confirm lung metastasis and no tumor metastasis in other sites. The intraperitoneal inoculation model Tumor cells formed multiple metastases in the abdominal cavity, and the tumor cells continued to proliferate. Imaging in vitro 6 weeks after inoculation revealed multiple metastases in the parietal peritoneum and visceral peritoneum. No metastasis was found in various organs and beyond the abdominal cavity. Conclusions Two models of metastatic nude mice were established, and the application of bioluminescence imaging to the tracking and monitoring of deep tumors.