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现已发现,除宿主基因型和年令外,接种途径、毒株及其传代史,都会影响各株鼠肝炎病毒的致病性和感染情况。同一毒株,如JHM株,以不同途径接种不同品系小鼠所产生的病变、病毒复制部位以及组织中病毒滴度均有差异。给刚断奶的BALB/cByJ小鼠鼻内接种MHV—JHM病毒,死亡率很高,而同样接种随机繁育的CF1小鼠,则无临床症状。此处所报道的工作的目的之一,是要确定经鼻接种致死量MHV—JHM后,该病毒在敏感鼠和抵抗鼠的复制部位。另外,本实验室的体外实验表明:各野生型MHV诱生干扰素的能力均很差,但对干扰素敏感,故本实验还要确定在天然宿主身上是否也有同样现象。第三个目的,是要确定预先感染MHV—JHM能否保护小鼠抵抗致死剂量的同型和同种异型病毒(MHV—3)的感染。
It has been found that in addition to the host genotype and year, the route of vaccination, the strain and its passage history, will affect the pathogenicity and infection of each rat hepatitis virus. The same strain, such as JHM strain, inoculated with different strains of different strains of mice lesions, viral replication sites and the organization of the virus titer are different. Inoculation of MHV-JHM virus intranasally to newly weaned BALB / cByJ mice resulted in a high mortality rate, while no clinically significant symptoms were observed in randomly-inoculated CF1 mice. One of the goals of the work reported here is to determine the virus’s susceptibility to the murine and replication-resistant parts of the mouse after intranasal inoculation of the lethal dose of MHV-JHM. In addition, in our laboratory, in vitro experiments showed that the ability of each wild-type MHV to induce interferon is very poor, but sensitive to interferon. Therefore, this experiment also needs to determine whether the same phenomenon occurs in the natural host. A third objective is to determine whether preinfection with MHV-JHM protects mice against lethal doses of the isotype and allogeneic virus (MHV-3).