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目的阐明光叶合欢的抗癌活性成分。方法以细胞凋亡诱导和细胞周期抑制活性为抗癌指标,采用柱色谱、薄层色谱等分离技术并结合流式细胞术,跟踪分离活性成分,利用谱学方法鉴定化学结构。结果从光叶合欢中分离鉴定了布木柴胺K(1)、(+) - 9 -去甲布木柴胺K(2 )、邻苯二甲酸二丁酯(3)、邻苯二甲酸二(2 乙基 己基)酯(4)和β-谷甾醇(5 )。化合物1～4显著诱导K5 6 2细胞凋亡,在低浓度时还将细胞周期抑制在G0 /G1期。结论化合物2为新化合物,化合物1～5为首次从该植物中分离得到,其中1～4为该植物活性成分的首例报道。
Objective To elucidate the anticancer active ingredients of Albizia julibrissin. Methods Apoptosis induction and cell cycle inhibition activity were used as anti-cancer indicators. Separation techniques such as column chromatography and thin-layer chromatography were used in combination with flow cytometry to trace the active components. The chemical structures were identified by spectroscopic methods. Results Bambuzine K(1), (+)-9-Methylcarbanimidin K(2), dibutyl phthalate (3), phthalate di(isobutyrate) were isolated and identified from A. luminifera. 2 ethylhexyl) ester (4) and β-sitosterol (5). Compounds 1-4 significantly induced apoptosis in K562 cells, and at low concentrations also inhibited the cell cycle in G0/G1 phase. Conclusion Compound 2 is a new compound. Compounds 1 to 5 were isolated from the plant for the first time. Among them, 1-4 were the first reported examples of the active components of the plant.