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目的:优选平性人参炮制工艺。方法:采用栀子、黄连、知母分别制备平性人参;采用Bradford法测定蛋白含量;采用紫外分光光度法测定过氧化物酶(peroxydase,POD)、过氧化氢酶(catalase,CAT)和超氧化物歧化酶(superoxide dismutase,SOD)活力;采用单因素考察法,优选栀子制人参炮制工艺。结果:3种平性人参以栀子制人参抗氧化酶活力最高,其最佳炮制工艺为:物料比(人参∶栀子)1∶1,pH6.0,料液比1∶40,浸提2次,每次12 h。结论:3种平性人参均具有抗氧化酶活性,且以栀子制人参抗氧化酶活性最高;单因素试验筛选出的栀制人参炮制工艺合理、可行。
Objective: To optimize the flat ginseng processing technology. Methods: Geniposide was prepared by using Gardenia jelly, Rhizoma Coptidis, Rhizoma Anemarrhena as raw material. The protein content was determined by Bradford method. Peroxidase (POD), catalase (CAT) Superoxide dismutase (SOD) activity; using single factor method, preferably gardenia ginseng processing technology. Results: The anti-oxidase activities of ginseng and ginseng were the highest among the three kinds of ginseng. The optimal processing conditions were as follows: material ratio (ginseng: gardenia) 1: 1, pH 6.0, ratio of material to liquid 1:40, 2 times, each time 12 h. CONCLUSION: All three kinds of Ginseng have anti-oxidative enzyme activity, and the activity of Anti-oxidase of Ginseng is the highest. The process of Ginseng Ginseng selected by single factor test is reasonable and feasible.