视网膜中良好的血液循环与视网膜神经细胞的存活密切相关。在缺氧缺血条件下神经细胞的存活除了受其自身特性影响外,还受该细胞所处的微环境的调节。而血-视网膜屏障对调节视网膜的微环境,维持其稳态起重要作用。了解血-视网膜屏障的检测方法对研究血-视网膜屏障损伤在缺氧缺血性视网膜疾病的作用具有重要意义。本文着重介绍多种检测血-视网膜屏障的方法,为研究者检测血-视网膜屏障的损伤提供方法学资料。 Good blood circulation in the retina is closely related to the survival of retinal nerve cells. Under hypoxic-ischemic conditions, the survival of nerve cells in addition to its own characteristics, but also by the cells in the micro-environment regulation. However, the blood-retinal barrier plays an important role in regulating the microenvironment of the retina and maintaining its homeostasis. Understanding the blood-retinal barrier assay is important for studying the role of blood-retinal barrier damage in hypoxic-ischemic retinal diseases. This article focuses on a variety of methods for detecting blood-retinal barriers, providing methodological information for investigators to detect blood-retinal damage.