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目的研究用阳离子交换树脂提取拐枣七中总生物碱的工艺条件。方法以总生物碱的吸附量、解吸率和其中盐酸小檗碱的含量为考察指标,对5种不同型号的阳离子交换树脂进行评价,并考察最佳工艺参数。结果最佳提取工艺是:拐枣七药材粉碎成粗粉,加入10倍量的70%乙醇,浸泡2 h,加热回流提取3次,每次1 h。C008型阳离子交换树脂为最佳吸附树脂,其最大吸附量为0.65 g.g 1,4%氨水乙醇为洗脱溶剂,洗脱率为13.81%。结论 C008型阳离子交换树脂可用于提取拐枣七中总生物碱,该方法简便可行。
Objective To study the extraction conditions of total alkaloids from Callus jujube with cation exchange resin. Methods The adsorption and desorption rates of total alkaloids and the content of berberine hydrochloride were used as indicators to evaluate five different types of cation exchange resins, and the best process parameters were investigated. Results The best extraction process was as follows: Crude seven herbs were crushed into coarse powder, 10 times amount of 70% ethanol was added, soaked for 2 hours, and heated and refluxed for 3 times, each time for 1 hour. The C008 type cation exchange resin was the best adsorbent resin. The maximum adsorption capacity was 0.65 g.g. 1,4% ethanol ammonia as the elution solvent, and the elution rate was 13.81%. Conclusion C008 cation exchange resin can be used to extract total alkaloids from Callus radix sinensis. This method is simple and feasible.