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目的:分析MEK-7222型全自动血细胞分析仪计数血小板的影响因素。方法:使用MEK-7222型全自动血细胞分析仪在不同条件下对血小板进行测定,并在血小板直方图出现异常时采用手工镜检法计数,两者作对比分析。结果:采用MEK-7222型全自动血细胞分析仪测定血小板,放置10min血小板数显著高于即刻血小板数(P<0.05),之后10min~4h血小板数无显著性变化(P>0.05),放置8h后,血小板数显著低于放置10min血小板数(P<0.05)。采用手工镜检法测定血小板,即刻血小板数与放置10min~4h血小板数无显著性变化(P>0.05),放置8h后血小板数显著降低(P<0.05)。平均红细胞体积(MCV)>70fl时,血细胞分析仪与镜检法血小板计数结果比较,差异无统计学意义(P>0.05);MCV<70fl时,血细胞分析仪血小板计数显著高于镜检法计数(P<0.05)。结论:MEK-7222型全自动血细胞分析仪计数血小板的准确性与标本放置时间、平均红细胞体积的大小有关,必要时可进行重新采血或手工计数。
Objective: To analyze the influencing factors of MEK-7222 automatic blood cell analyzer counting platelets. Methods: MEK-7222 automatic hematology analyzer was used to measure platelet under different conditions and counted by manual microscopy when the platelet histogram appeared abnormal. The two were compared. Results: Platelets were measured by MEK-7222 automatic hematology analyzer. The number of platelets was significantly higher than the number of platelets after 10 minutes (P <0.05), but no significant changes after 10 minutes to 4 hours (P> 0.05) , The number of platelets was significantly lower than the number of platelets after 10min (P <0.05). Platelet count was measured by manual microscopy. There was no significant change in the number of platelets and the number of platelets after 10 minutes to 4 hours of exposure (P> 0.05). The number of platelets decreased significantly after 8 hours of storage (P <0.05). There was no significant difference between the hematology analyzer and the microscopic examination (P> 0.05) when the average volume of erythrocytes (MCV) was> 70 and the platelet count of the hematology analyzer was significantly higher than that of the microscopic examination (P <0.05). Conclusion: MEK-7222 automatic hematology analyzer counts platelet accuracy and specimen placement time, the average size of the volume of red blood cells, if necessary, re-blood or manual counting.