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切除10只雄猫的一侧L1~L5、L7~S2背根节(DRG)。保留L8背根为备用背根。电针刺激5只猫术侧L6背根分布区的足三里和悬钟、三阴交和伏兔两组穴位10天,另5只不针刺。术后第11天取脊髓背核组织,制备提取液,加入培养液。参照组以Hanks平衡盐溶液代替提取液。用不同的条件培养液培养鸡胚DRG。于培养24与48小时测定各DRG神经突起的长度,以同一批培养各组平均长度与参照组平均长度的比值衡量各组提取液对DRG神经突起生长的影响。组间比较发现,针刺手术侧组的平均比值明显大于非针刺手术侧组和非手术侧组,非针刺的求侧组又明显大于非手术侧组。提示,部分去背根传入猫的背核组织提取液,其促进神经突起生长的作用增强,电针刺激穴位能进一步提高其促神经突起生长的效应。
One side of 10 male cats was excised from L1 to L5 and L7 to S2 dorsal root ganglia (DRG). Keep the L8 dorsal root as a spare dorsal root. Electro-acupuncture stimulated the distribution of Zusanli and Xuanzhong in L6 dorsal root in 5 cats, and the acupoints in Sanyinjiao and Vuvu were both 10 days and the other 5 were not acupuncture. On the 11th postoperative day, the dorsal nucleus of the spinal cord was taken, and the extract was prepared and added into the culture fluid. Reference group Hanks balanced salt solution instead of the extract. Chicken embryo DRG was cultured in different conditions. The length of each DRG neurite was measured at 24 and 48 hours after culture. The effect of each extract on DRG neurite outgrowth was measured by the ratio of the average length of each group to the average length of the reference group. The comparison between groups showed that the average ratio of acupuncture group was significantly larger than that of non-acupuncture group and non-acupuncture group. The non-acupuncture group was significantly larger than non-acupuncture group. Tip, part of the dorsal root into the cat’s dorsal nucleus tissue extract, which enhances the role of neurite growth increased, acupuncture points can further stimulate their neurite outgrowth effect.