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目的:建立高效液相色谱法测定室尘中16种常见多环芳烃(PAHs)的最佳分离条件及测定的最佳荧光激发、发射波长和增益。方法:用吸尘器采集室尘,用丙酮超声提取室尘中的多环芳烃化合物,浓缩后用乙腈定容,乙腈和水为流动相,进行高效液相色谱梯度淋洗分离,程序波长荧光检测器检测。结果:除苊烯外的15种多环芳烃化合物在28 min内得到了很好的分离,在0.5～50 ng/ml范围内线性关系良好,测定的相对标准偏差为1.35%～5.89%,加标回收率为62.45%～110.36%。结论:该方法测定室尘中多环芳烃化合物快速简便、灵敏、重现性好。
OBJECTIVE: To establish the optimum conditions for the determination of 16 common PAHs in room dust by HPLC and to determine the best fluorescence excitation, emission wavelength and gain. Methods: Room dust was collected with a vacuum cleaner, polycyclic aromatic hydrocarbons (PAHs) in room dust were extracted with acetone and concentrated. After concentration, acetonitrile, water and acetonitrile were used as mobile phases. High performance liquid chromatography (HPLC) Detection. RESULTS: Fifteen polycyclic aromatic hydrocarbons (PAHs) other than camphene were well separated in 28 min. The calibration curves were linear within the range of 0.5-50 ng / ml. The relative standard deviations (RSDs) were 1.35% -5.89% The standard recovery was 62.45% ~ 110.36%. Conclusion: This method is rapid, simple, sensitive and reproducible for the determination of PAHs in room dust.