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杉木CAD片段为信息探针,利用序列拼接方法获得了738bp的cDNA序列.利用MEGA4.0软件对不同进化层次的代表植物相应CAD基因进行比对,构建系统进化树.利用生物信息学方法分析了杉木CAD基因的结构与功能,其中包括核苷酸序列的组成、ORF识别、CpG岛、限制性酶切位点分析、卷曲螺旋、亲水性、糖基位点及二级结构.结果表明:杉木与铁杉的CAD基因具有99.00%的同源性;该序列在88～737bp片段上有一个开放性阅读框,该序列编码的是一个碱性蛋白,亲水性较强,疏水性较弱,信号肽序列为第1位～第11位,二级结构以α-螺旋(27.50%)与不规则盘绕(41.50%)为主,延伸链(22.50%)也是该蛋白的重要结构元件,β转角区域仅为8.50%.
The CAD fragment of Chinese fir was an information probe, and a 738 bp cDNA sequence was obtained by using the sequence stitching method. The phylogenetic tree was constructed by using MEGA4.0 software to compare the corresponding CAD genes of representative plants at different evolutionary levels. The bioinformatics method was used to analyze The structure and function of Chinese fir (CAD) gene, including the composition of nucleotide sequence, ORF recognition, CpG island, restriction analysis, coiled coil, hydrophilicity, glycosylation site and secondary structure, The CAD gene of Chinese fir and Hemlock had 99.00% homology. The open reading frame (ORF) of the 88 ~ 737bp fragment encoded a basic protein with strong hydrophilicity and poor hydrophobicity , The signal peptide sequence is from the 1st to the 11th, the secondary structure is dominated by α-helix (27.50%) and irregular coil (41.50%), and the extended chain (22.50%) is also an important structural element of β The corner area is only 8.50%.