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目的分析p57kip2和PCNA蛋白在西宁地区食管鳞癌组织中的表达,探讨其在食管鳞癌发生发展中的意义。方法选取60例食管鳞癌及其相对应的31例癌旁正常组织标本,应用免疫组化方法检测p57kip2和PCNA蛋白,并结合临床病理特点分析。结果p57kip2在食管鳞癌中的表达率为28.33%,低于癌旁正常组织的表达率(51.61%),并与食管鳞癌的分化程度、浸润深度有关,差异有统计学意义(P<0.05)。PCNA在食管鳞癌中的表达率为81.67%,高于癌旁正常组织的表达率(61.29%),与食管鳞癌的分化程度、浸润深度、淋巴结转移有关,差异有统计学意义(P<0.05)。p57KIP2与PCNA蛋白的表达呈负相关(rs=-0.611,P<0.01)。结论食管鳞癌组织中p57kip2低表达与PCNA的高表达共同参与食管癌的发生、发展。联合检测可作为预测食管鳞癌恶性程度和预后的重要指标。
Objective To analyze the expression of p57kip2 and PCNA protein in esophageal squamous cell carcinoma of Xining and to explore its significance in the occurrence and development of esophageal squamous cell carcinoma. Methods Sixty esophageal squamous cell carcinomas and 31 corresponding normal tissues were selected for immunohistochemistry. The expressions of p57kip2 and PCNA were detected by immunohistochemistry and analyzed with clinicopathological features. Results The positive rate of p57kip2 in esophageal squamous cell carcinoma was 28.33%, which was lower than that in adjacent normal tissues (51.61%), which was related to the differentiation degree and depth of invasion of esophageal squamous cell carcinoma (P <0.05) ). The positive rate of PCNA in esophageal squamous cell carcinoma was 81.67%, which was significantly higher than that in adjacent normal tissues (61.29%), which was related to the differentiation, depth of invasion and lymph node metastasis of esophageal squamous cell carcinoma (P < 0.05). The expression of p57KIP2 and PCNA protein were negatively correlated (rs = -0.611, P <0.01). Conclusion The low expression of p57kip2 and the high expression of PCNA in esophageal squamous cell carcinoma are involved in the occurrence and development of esophageal cancer. Joint detection can be used as an important index to predict the malignancy and prognosis of esophageal squamous cell carcinoma.