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目的:探讨p57、p53和p63蛋白在鉴别诊断水肿性流产(HA),部分性葡萄胎(PHM)和完全性葡萄胎(CHM)的价值。方法:采用免疫组织化学染色法检测HA(n=21)、PHM(n=28)和CHM(n=32)石蜡组织切片中p57、p53和p63的表达。结果:HA、PHM和CHM组织中,p57蛋白的阳性表达率分别为90.5%(19/21)、100%(28/28)和0.0%(0/32),PHM和CHM组间有明显差异(P<0.01);p53蛋白的阳性表达率分别为28.3%(5/21)、71.4%(20/28)和87.5%(28/32),HA、PHM组相比也有明显差异(P<0.01);p63蛋白的阳性表达率分别为81.0%(17/21)、89.3%(25/28)和87.5%(28/32),各组间均无统计学差异。结论 :以常规石蜡切片的病理形态特征为基础,结合免疫组化检测p57、p53和p63的结果,对鉴别水肿性流产和葡萄胎具有重要价值。
Objective: To investigate the value of p57, p53 and p63 proteins in the differential diagnosis of edema (HA), partial hydatidiform mole (PHM) and complete hydatidiform mole (CHM). Methods: Immunohistochemical staining was used to detect the expression of p57, p53 and p63 in paraffin sections of HA (n = 21), PHM (n = 28) and CHM (n = 32). Results: The positive rates of p57 protein in HA, PHM and CHM tissues were 90.5% (19/21), 100% (28/28) and 0.0% (0/32), respectively, with significant differences between PHM and CHM groups (P <0.01). The positive expression rates of p53 protein were 28.3% (5/21), 71.4% (20/28) and 87.5% (28/32) respectively. There was also significant difference between HA and PHM groups (P < 0.01). The positive expression rates of p63 protein were 81.0% (17/21), 89.3% (25/28) and 87.5% (28/32) respectively. There was no significant difference among the three groups. Conclusion: Based on the pathological features of conventional paraffin sections, combined with the results of immunohistochemical detection of p57, p53 and p63, it is of great value in the identification of edematous abortion and hydatidiform mole.