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目的:研究强精煎对实验小鼠睾丸Bcl-2、Bax蛋白表达及Bcl-2/Bax比值的影响。方法:80只成年健康雄性小鼠随机分成正常组、模型组、强精煎组和黄精赞育胶囊组各20只。除正常组外,其余3组采用环磷酰胺腹腔注射建立小鼠生精障碍模型,造模结束后第1天开始正常组和模型组灌胃给蒸馏水,其余两组分别给强精煎冲剂、黄精赞育胶囊混悬液,连续30天。结果:与模型组相比,正常组、强精煎组和黄精赞育胶囊组睾丸生殖细胞凋亡基因Bax蛋白表达率较低(P<0.01)、Bcl-2蛋白表达率较高(P<0.01)、细胞内Bcl-2/Bax比值较高(P<0.01),正常组、强精煎组和黄精赞育胶囊组各组间比较睾丸生殖细胞中Bax、Bcl-2蛋白表达及Bcl-2/Bax比值相近(P>0.05)。结论:强精煎治疗小鼠睾丸生精障碍的疗效与Bcl-2、Bax蛋白表达率及Bcl-2/Bax比值密切相关。
Objective: To study the effect of Qiangjingjian on Bcl-2 and Bax protein expression and Bcl-2/Bax ratio in testicles of experimental mice. METHODS: Eighty adult healthy male mice were randomly divided into normal group, model group, Qiangjing decoction group and Huangjing Zanyu capsule group. In addition to the normal group, the other three groups were treated with cyclophosphamide intraperitoneally to establish a mouse spermatogenic barrier model. On the first day after the completion of the model, the normal group and the model group were given distilled water by gavage, and the other two groups were given Qiang Jingjian Granules. Huang Jing Zanyu capsule suspension for 30 consecutive days. RESULTS: Compared with the model group, the expression rate of apoptosis gene Bax protein in testis germ cells was lower in normal group, qiangjingjian group and Huangjingzanyu capsule group (P<0.01), and the expression rate of Bcl-2 protein was higher (P< 0.01). The ratio of intracellular Bcl-2/Bax was higher (P<0.01). The Bax, Bcl-2 protein expression and Bcl- in testicular germ cells were compared between the normal group, the strong fried group and the Huangjing Zanyu capsule group. The 2/Bax ratio was similar (P > 0.05). Conclusion: The efficacy of strong spermatogenesis in the treatment of testicular spermatogenic disorders in mice is closely related to the expression of Bcl-2 and Bax protein and Bcl-2/Bax ratio.