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选用高特异性单克隆抗 GL P- 1- IR(7- 36 )酰胺抗体 ,用免疫组织化学方法估价了 GL P- 1- IR细胞在鼠、猪和人胎儿肠道中的分布。结果发现 :在各段肠管都可见到 GL P- 1- IR细胞 ;GL P- 1- IR细胞散在于肠腺的柱状上皮细胞之间 ,多位于肠腺的基底部 ,呈开放型内分泌细胞形态 ,即 L 细胞 (EG细胞 ) ;不同种属间 GL P-1- IR细胞密度不同 ,但分布规律相同 ,即自小肠和大肠的近端向远端逐渐增大。结果表明 ,分泌 GL P- 1的 L 细胞 (GL P- 1- IR细胞 )主要分布在肠道远端 ,这提示 ,远端肠道中大量存在的 L 细胞可以接受在近端胃肠道未被吸收的营养物质的刺激 ,使 L 细胞释放 GL P- 1入血
The distribution of GLP-1-IR cells in the intestine of mice, pigs and human fetuses was evaluated by immunohistochemistry using highly specific monoclonal anti-GLP-1-IR (7-36) amide antibody. The results showed that: GL P-1-IR cells were observed in the intestines of various stages; GL P-1-IR cells scattered between the columnar epithelial cells of the gut gland and mostly located in the basal part of the gut gland with an open endocrine cell morphology , That is, L cells (EG cells). The density of GL P-1-IR cells varied among different species but the distribution was the same, that is, gradually increased from proximal to distal of small intestine and large intestine. The results show that GL-1-secreting L cells (GLP-1-IR cells) are predominantly located in the distal gut which suggests that the presence of large numbers of L cells in the distal gut may be tolerated in the proximal gastrointestinal tract Stimulation of absorbed nutrients allows L cells to release GL-1 into the blood stream