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目的探讨早产儿脐带血中巨细胞病毒(CMV)、单纯疱疹病毒Ⅰ(HSV1),单纯疱疹病毒Ⅱ(HSV2)的感染与早产的相关性。方法采用实时荧光定量PCR(real-time PCR),对我院2015年6月1日-2016年12月31日出生的89例早产儿(研究组)和235例正常足月儿(对照组)脐带血进行检测。结果 89例早产儿中CMV-DNA检出10例,阳性率为11.24%,HSV1-DNA检出5例,阳性率为5.62%,HSV2-DNA检出4例,阳性率为4.49%。235例足月儿中CMV-DNA检出4例,阳性率为1.70%,HSV1-DNA检出2例,阳性率为0.85%,HSV2-DNA检出0例,阳性率为零。早产儿的CMV、HSV1、HSV2的感染率均高于足月儿,差异比较有统计学意义(P<0.05)。结论脐带血中CMV、HSV1、HSV2感染与早产的发生有明显相关性;且通过real-PCR方法检测较传统的检测方法敏感性强,准确度高。
Objective To investigate the correlation between cord blood cytomegalovirus (CMV), herpes simplex virus (HSV1) and herpes simplex virus (HSV2) in premature infants and preterm labor. Methods Eighty-eight preterm infants (study group) and 235 normal term infants (control group) were born in our hospital from June 1, 2015 to December 31, 2016. Real-time PCR Blood testing. Results The positive rate of CMV-DNA in 89 preterm infants was 11.24%. The positive rate of HSV1-DNA was 5.62%. The positive rate of HSV2-DNA was 4.49%. In 235 full-term infants, CMV-DNA was detected in 4 cases, the positive rate was 1.70%, HSV1-DNA was detected in 2 cases, the positive rate was 0.85%, HSV2-DNA was detected in 0 cases, the positive rate was zero. The infection rates of CMV, HSV1 and HSV2 in preterm infants were higher than those in term infants, the difference was statistically significant (P <0.05). Conclusion The infection of CMV, HSV1 and HSV2 in umbilical cord blood has a clear correlation with the occurrence of preterm labor. And the detection by real-PCR method is more sensitive and accurate than the traditional detection methods.