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目的:通过改变细胞培养时铁状态,观察TPA诱导K562细胞分化过程中细胞铁对K562细胞生长分化的影响,探讨铁与白血病细胞诱导分化的关系。方法:应用体外细胞培养技术,观察不同铁水平K562细胞生长抑制率;用非特异性酯酶染色观察细胞生长分化情况。结果:在TPA诱导K562细胞分化过程中TPA组和TPA+Fe组K562细胞生长抑制率分别为8.67%、6.01%,而TPA-Fe组98.88%。对照组及TPA-Fe组K562细胞NSE染色阳性率为15.5%和13%,以弱阳性为主,氟化钠抑制率分别为16.13%和19.23%;TPA、TPA+Fe组K562细胞NSE染色呈阳性和强阳性,阳性率分别为86%、88%,且这两组K562细胞氟化钠抑制率分别为58.72%、61.93%。结论:铁剥夺可明显抑制K562细胞生长、阻止TPA诱导K562细胞分化,故铁剥夺剂(DFO)可能作为一种辅助抗癌药用于白血病的化疗,但由于它能阻止白血病细胞的分化,故不宜用于白血病的诱导分化治疗。
OBJECTIVE: To investigate the effects of iron on the growth and differentiation of K562 cells induced by TPA-induced differentiation of K562 cells and to explore the relationship between iron and the differentiation of leukemia cells by changing the state of iron during cell culture. Methods: In vitro cell culture technique was used to observe the growth inhibition rate of K562 cells with different iron levels. Cell growth and differentiation were observed by nonspecific esterase staining. Results: The growth inhibition rates of K562 cells in TPA group and TPA + Fe group were 8.67% and 6.01%, respectively, in the TPA-induced K562 cell differentiation and 98.88% in the TPA-Fe group. The positive staining rate of NSE in K562 cells in control group and TPA-Fe group was 15.5% and 13%, respectively. The weak positive staining and the sodium fluoride inhibition rates were 16.13% and 19.23%, respectively. The NSE staining of K562 cells in TPA and TPA + Fe group was Positive and strong positive, the positive rates were 86%, 88%, respectively, and the inhibition rates of sodium fluoride in K562 cells in these two groups were 58.72% and 61.93%, respectively. Conclusion: Iron deprivation can significantly inhibit the growth of K562 cells and prevent the differentiation of K562 cells induced by TPA. Therefore, iron-deprivation agent (DFO) may be used as a complementary anti-cancer drug for the chemotherapy of leukemia. However, DFR can prevent the differentiation of leukemia cells Not suitable for the differentiation of leukemia treatment.