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以红酵母静息细胞为生物催化剂,在由两种不同浊点的表面活性剂组成的浊点系统中对3,5-二(三氟甲基)苯乙酮进行生物转化,以生产3,5-二(三氟甲基)苯乙醇。分别考察了浊点系统对细胞生长和转化过程的影响。在细胞培养阶段表面活性剂Triton X-114(TX-114)能够大大提高细胞对毒性底物的耐受能力。表面活性剂浓度5%,底物浓度6 l/ml时,浊点系统的产物浓度为5.47 mg/ml,转化率为80.5%,分别比两相系统提高91.3%和78.1%。细胞经4次循环使用,活性仍保持80%以上。
3,5-Bis (trifluoromethyl) acetophenone was biotransformed in a cloud point system consisting of two surfactants of different cloud points using the resting cells of Rhodotorula as biocatalysts to produce 3, 5-bis (trifluoromethyl) benzeneethanol. The effects of cloud point system on cell growth and transformation were investigated. Surfactant Triton X-114 (TX-114) can greatly enhance the tolerance of cells to toxic substrates during cell culture. When the surfactant concentration was 5% and the substrate concentration was 6 l / ml, the cloud point system had a product concentration of 5.47 mg / ml and a conversion rate of 80.5%, which was 91.3% and 78.1% higher than the two-phase system. After 4 cycles of cells, the activity remained above 80%.