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[目的]建立同时测定尿样中4种多环芳烃代谢产物的反相高效液相色谱-荧光检测方法。[方法]样品经酶解,用C18固相萃取柱净化、氮吹浓缩后,采用高效液相色谱柱分离、荧光检测器检测,以外标法定量。[结果]4种多环芳烃代谢产物标准曲线的线性良好;方法检出限为0.015～0.086μg/L;尿样低、中、高3个水平的平均加标回收率为75.23%～127.5%,相对标准偏差为4.43%～9.87%。用所建立的方法成功测定了正常人群20份尿样中的4种多环芳烃代谢产物。[结论]本方法具有准确、灵敏、重现性好的特点,适合于尿样中4种多环芳烃代谢产物的同时测定。
[Objective] To establish a reversed-phase high performance liquid chromatography-fluorescence detection method for simultaneous determination of four PAHs metabolites in urine. [Method] The samples were digested with C18 solid phase extraction column, purged with nitrogen, then separated by high performance liquid chromatography, detected by fluorescence detector and quantified by external standard method. [Result] The calibration curves of four kinds of PAHs were linear. The detection limits were 0.015 ~ 0.086 μg / L. The average recoveries of the three kinds of PAHs were 75.23% ~ 127.5% , The relative standard deviation was 4.43% ~ 9.87%. Using the established method, we successfully measured four PAHs metabolites in 20 urine samples of normal population. [Conclusion] The method is accurate, sensitive and reproducible. It is suitable for the simultaneous determination of four kinds of PAHs metabolites in urine samples.