腺病毒用于人类基因治疗有许多优点,亦有很高的潜在应用价值。但目前所用腺病毒有三方面的缺点:①插入容量小,限8kb以下;②载体病毒蛋白的表达可能引起被感染组织的炎性反应;③被转基因的表达时间短。为此作者发展了一种新的移去所有的病毒编码序列腺病毒载体,外源插入容量增 Adenovirus for human gene therapy has many advantages, but also a high potential value. However, adenovirus currently used has three disadvantages: ① small insertion capacity, limited to 8kb or less; ② the expression of the carrier virus protein may cause inflammatory reaction in infected tissues; ③ transgenics expression time is short. To this end, the authors developed a new adenovirus vector that removes all viral coding sequences, increasing the amount of exogenous insert