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本文对原产我国的优良桃砧木—山桃[Prunus davidiana(Carr.)Franch]进行了茎尖培养的研究。山桃茎尖在G培养基上增殖与生长最好。适宜的激素浓度为(毫克/升)BA1.0、IBA0.5,若再加入GA0.1,可明显促进新梢的生长。附加BA、IBA各0.1的G和1/2G大量元素培养基适于继代培养。培养出的新梢在1/2MS培养基中附加0.1毫克/升NAA生根率高,在新梢基部削伤和暗培养可提高生根率。小植株已成功移栽入土。
In this paper, the shoot tip culture of prunus davidiana (Carr.) Franch, a fine peach rootstock rooted in China, was studied. Prunus mume shoot propagation and growth in G medium best. The appropriate hormone concentration (mg / L) BA1.0, IBA0.5, if added GA0.1, can significantly promote the growth of shoots. Additional BA, IBA 0.1 G and 1 / 2G macronutrient media are suitable for subculture. The cultured shoots had a high rooting rate of 0.1 mg / l NAA in 1 / 2MS medium, and the rooting rate could be improved by cutting and darkening the shoots at the shoot tip. Plantlets have been successfully transplanted into the soil.