论文部分内容阅读
植物杂交F1的株高一般都表现出明显的杂种优势,但其形成的分子机理迄今尚未阐述清楚.本研究以按照NCⅡ遗传交配设计配制的16个杂交组合为材料,在田间株高性状杂种优势测定的基础上,采用实时定量PCR技术检测了赤霉素代谢及其调控相关基因在杂交种与亲本抽穗期穗下第1节中的表达情况,并且与株高杂种优势进行了相关分析.结果表明,所有杂交组合的株高和第1节均表现出明显的杂种优势,但因杂交组合和株高性状不同而存在很大的差异.分析发现,第1节与株高的中亲杂种优势之间的相关性达到了显著水平(r=0.56,P<0.05),说明该节对小麦株高杂种优势的形成有重要的贡献.实时定量PCR表达分析结果显示,赤霉素代谢及调控相关基因的表达优势因杂交组合不同而存在明显的差异,但第1节杂种优势与KS,GA3ox2-1,GA20ox2,GA20ox1D,GA-MYB和GID1-1基因的表达优势呈显著或极显著正相关,而与GAI和GA2ox-1基因的表达优势呈极显著负相关,这与我们最近提出的小麦株高杂种优势形成的赤霉素分子调控模型相吻合,说明赤霉素代谢及调控相关基因的表达改变与株高杂种优势的形成有重要关系.
The plant height of plant hybrid F1 generally showed obvious heterosis, but the molecular mechanism of its formation has not been clarified so far.In this study, 16 hybrid combinations were designed according to NCⅡ genetic mating design, Based on the results of real-time quantitative PCR, we detected the expression of gibberellin metabolism and its regulation genes in the ear under the heading stage of hybrids and parents, and analyzed the correlation with plant height heterosis. The results showed that all the hybrid combinations showed significant heterosis in plant height and section 1, but there was a big difference in hybrid combinations and plant height traits.The analysis showed that the heterosis in section 1 and plant height (R = 0.56, P <0.05), indicating that this section has an important contribution to the formation of heterosis in plant height.Results of quantitative real-time PCR showed that the metabolism and regulation of gibberellin However, the dominant heterosis in section 1 was significantly or extremely significantly different from those in KS, GA3ox2-1, GA20ox2, GA20ox1D, GA-MYB and GID1-1 , But negatively correlated with the GA1 and GA2ox-1 gene expression. This is in line with our recently proposed GA molecular regulation model of plant height and heterosis, indicating that the GA3 and GA2ox- The change of expression has an important relationship with the formation of heterosis.