目的　构建 MC1 4 8腺病毒重组体。方法　从传染性软疣病毒基因组中克隆 MC1 4 8基因 ,产物测序后将之插入柯斯质粒 ,然后与 DNA- TPC共转染进 2 93细胞 ,同源重组构建腺病毒重组体。浓度梯度法测定病毒滴度。结果　成功构建 Ad- MC1 4 8腺病毒重组体 ,经测定滴度达 3.5 6× 1 0 9(pfu/ml)。结论　本方法建立为研究 MC1 4 8基因转染对趋化因子的拮抗作用奠定基础 Objective To construct MC1 4 8 recombinant adenovirus. Methods MC1 48 gene was cloned from the genome of molluscum contagiosum. The product was inserted into cosmid after sequencing, and then co-transfected into 293 cells with DNA-TPC. Recombinant adenovirus was constructed by homologous recombination. Determination of virus titer by concentration gradient method. Results Ad-MC1 4 8 recombinant adenovirus was successfully constructed and its titer was 3.5 6 × 109 (pfu / ml). Conclusion This method was established to provide a basis for studying the antagonism of chemokines by MC1 4 8 gene transfection