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1.供试卵:是在土当归出芽处理后从废弃的残留植株上由羽化的成虫产出.即把2对羽化的雌、雄成虫放进24厘米×36厘米×30厘米的金属饲养笼中,供给容易产卵的八角金盘叶柄,以后把所产的卵从叶柄上剥下收集在器皿中,置于4%次氯酸20倍液和70%酒精混合液中分别浸渍数十秒钟,尔后用蒸馏水冲洗.为防止孵化幼虫自相残杀,在长21厘米、宽11厘米、厚3毫米的丙烯树脂板上,钻一直径为8毫米的孔,做成孵化器(图A).在孔的底侧,垫上浸过蒸馏水的滤纸.放进灭菌卵后,用同样大的玻璃板放在两面,并用三明治状橡皮轮相隔.
1. The test eggs were produced from eclosion adults on the abandoned plant after sprouting from the soil, that is, two pairs of females and males of the females were put into a 24 cm × 36 cm × 30 cm metal cage , To provide easy to spawn oviposition of gold leaf petiole, after the eggs produced petiole peeled off collected in the vessel, placed in 4% hypochlorous acid 20 times and 70% alcohol solution were immersed for tens of seconds Afterwards, rinsed with distilled water, an incubator (Figure A) was drilled with a hole of 8 mm in diameter on an acrylic resin board 21 cm long by 11 cm wide by 3 mm thick to prevent the larvae from killing each other. On the underside of the wells, pad the filter paper soaked in distilled water, put the sterilized eggs, place the same large glass plates on both sides, and separate them with sandwich-type rubber wheels.