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克隆茶树Ⅱ型核糖体失活蛋白基因Cs RIPI和Cs RIP2,能够参与茶树的防卫反应,加深对Cs RIPs基因转录调控以及RIPs的生物学功能理解。以凫早2号的幼苗为实验材料,对茶树Ⅱ型核糖体失活蛋白基因Cs RIPI和Cs RIP2进行了研究,其在营养钵生长了2 a,分析了Cs RIP基因克隆与序列、Cs RIP因编码产物的系统进化树、Cs RIP基因在不同组织中的表达等,并对Cs RIP基因编码产物的结构域分析和同源性进行了比较。
Cloning of the type II ribosome inactivating protein gene Cs RIPI and Cs RIP2 of tea plant can participate in the defense response of tea plant and deepen the understanding of the transcriptional regulation of Cs RIPs gene and the biological function of RIPs. The seedlings of Zaozao 2 were used as experimental material to study the expression of Cs RIPI and Cs RIP2, a type Ⅱ ribosome inactivation protein gene of tea plant, which was grown in a nutrient bowl for 2 years. The cloning and sequence of Cs RIP gene were analyzed. Due to the phylogenetic tree of the encoded products, the expression of Cs RIP gene in different tissues, etc., the domain analysis and homology of the encoded products of Cs RIP gene were compared.