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目的探讨3起同地连续发生的肠炎沙门菌食物中毒分离株之间的分子流行病学关系。方法参照GB 4789.4—2010和WS/T 13—1996的方法进行病原分离培养,对检出菌株进行表型鉴定;采用WHO推荐的改良K-B法对检出菌株进行抗生素敏感试验;采用脉冲场凝胶电泳(PFGE)分型方法对检出菌株进行分子分型和流行病学特征分析。结果 144份粪便及肛拭样本中检出23株肠炎沙门菌,其中3株从冷菜厨师肛拭中检出;54份留样食物中检出4株肠炎沙门菌(均为冷菜);共计27株菌。血清抗原式均符合[9:g,m:—];生物学性状和药敏试验结果一致;PFGE带型完全一致。结论这3起食物中毒由肠炎沙门菌污染引起;PFGE技术适用于菌株鉴定和溯源,以实现追踪传染源的目的。
Objective To investigate the molecular epidemiological relationship among three Salmonella enteritidis food poisoning isolates that occurred continuously in the same place. Methods According to the method of GB 4789.4-2010 and WS / T 13-1996, the pathogen was isolated and cultured, and the phenotypes of the tested strains were identified. The antibiotic susceptibility test of the tested strains was carried out by the modified KB method recommended by the WHO. The pulsed field gel Electrophoresis (PFGE) typing method for the detection of strains of molecular typing and epidemiological characteristics. Results A total of 23 Salmonella enteritidis strains were detected in 144 samples of feces and anal swabs, of which 3 were detected in anal swabs of cold-cooks. Four Salmonella enteritidis were detected in 54 samples (all were cold dishes) A total of 27 strains. Serum antigens are in line with the [9: g, m: -]; biological properties and susceptibility test results; PFGE band type exactly the same. Conclusion The three food poisonings caused by Salmonella enteritidis contamination; PFGE technology for identification and traceability of the strain, in order to achieve the purpose of tracking the source of infection.