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目的:对HBV血清标记物不同组合模式与HBV-DNA定量结果进行对比分析。方法:采用ELISA方法检测乙肝病毒血清标记物,荧光定量PCR检测HBV-DNA,比较二者关系。结果:HBsAg、HBeAg、HBcAb阳性组、HB-sAg、HBeAg阳性组及HBsAg、HBsAb、HBeAg、HBcAb阳性组HBV-DNA检出率和平均水平含量均较高。结论:荧光定量PCR检测HBV-DNA能准确反映体内HBV真实感染和复制情况,同时进行HBV血清标记物的检测与HBV-DNA荧光定量PCR的检测,更有利于HBV临床感染上的诊断、对治疗方案的选择及疗效评价。
Objective: To compare the different combinations of HBV serum markers and HBV-DNA quantitative results. Methods: Serum markers of hepatitis B virus were detected by ELISA and HBV-DNA by fluorescence quantitative PCR. The relationship between them was compared. Results: The detection rate and average level of HBV-DNA in HBsAg, HBeAg, HBcAb-positive group, HB-sAg, HBeAg-positive group and HBsAg, HBsAb, HBeAg and HBcAb-positive group were higher. Conclusion: The detection of HBV-DNA by fluorescence quantitative PCR can accurately reflect the true infection and replication of HBV in vivo, meanwhile, the detection of HBV serum markers and HBV-DNA fluorescence quantitative PCR are more beneficial to the diagnosis of HBV clinical infection. Choice of program and evaluation of curative effect.