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用丁酸钠 (NaBu)诱导了人胚肺二倍体成纤维细胞凋亡 (2BS) ,检测其诱导过程中凋亡相关基因的表达变化 ,结果表明 ,p2 1WAF1的表达在凋亡发生前即有明显下降 ,并持续至凋亡发生时 ,bcl 2的表达仅在凋亡发生时有所下降 ,c myc和c fos的表达有所上升 ,而 p5 3和HER 2的表达无明显变化 .用稳定转染了不同长度p2 1WAF1启动子片段和下游绿色荧光蛋白 (GFP)报告基因的 2BS WP系列细胞进一步研究发现 ,其GFP的表达水平在NaBu诱导过程中下降 ,主要调控区域为 p2 1WAF1启动子的TATAbox上游 0～ - 80 0bp .说明NaBu诱导的人胚肺二倍体成纤维细胞凋亡与p2 1WAF1启动子的转录活性下降与密切相关 ,并且可能不依赖于p5 3.
The apoptosis of diploid fibroblasts (2BS) induced by sodium butyrate (NaBu) was detected and the expression of apoptosis-related genes was detected during the induction. The results showed that the expression of p21WAF1 was up-regulated Decreased significantly and persisted till the onset of apoptosis, the expression of bcl 2 decreased only at the time of apoptosis, the expression of c myc and c fos increased, but the expression of p 5 3 and HER 2 did not change significantly 2BS WP cells stably transfected with p2 1WAF1 promoter fragment and downstream green fluorescent protein (GFP) reporter gene were further studied and found that the expression level of GFP decreased during the induction of NaBu, and the main regulatory region was p2 1WAF1 promoter Of the upstream of TATAbox 0 ~ -80 0bp, indicating that NaBu-induced apoptosis in human embryonic lung diploid fibroblasts is closely related to the decrease of transcriptional activity of p2 1WAF1 promoter and may not depend on p5 3.