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本文报道测定16种抗卫氏并殖吸虫(Pw)囊蚴和成虫单克隆抗体(McAb)的同型及其交叉反应。其中,抗Pw成虫McAb A_3D_3为IgM,k型。应用Dot-ELISA方法,以McAb A_3D_3检测痰卵阳性患者21例和痰卵阴性疑似患者63例血清循环抗原(CAg)),结果痰卵阳性患者CAg阳性率为100％(21/21),痰卵阴性疑似患者CAg阳性率为85.7％(54/63);同法检测45例日本血吸虫病、55例华支睾吸虫病、24例丝虫病、49例肠线虫感染、31例肺结核患者以及93例健康人对照血清均为阴性。结果表明,McAb A_3D_3的特异性及敏感性强,因而在Pw活动性感染和Pw病的早期诊断上均具有应用和推广前景。
This article reports the determination of 16 isoforms of Paw Ehrlichiae (Pw) metacercariae and adult monoclonal antibodies (McAbs) and their cross-reactions. Among them, anti-Pw adult McAb A_3D_3 is IgM, k type. The results showed that the positive rate of CAg was 100% (21/21) in sputum-positive patients and sputum-positive sputum was detected in 21 sputum-egg-positive patients and 63 sputum-egg-negative suspected patients by Dot-ELISA. The positive rate of CAg was 85.7% (54/63) in egg negative suspicious patients. The same method was used to detect 45 cases of Schistosoma japonicum, 55 cases of Clonorchiasis, 24 cases of filariasis, 49 cases of intestinal nematode infection, 31 cases of pulmonary tuberculosis and 93 cases of healthy control serum were negative. The results showed that the specificity and sensitivity of McAb A_3D_3 are strong, so it has the potential of application and promotion in active infection of Pw and early diagnosis of Pw.