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目的检测胃腺癌、印戒细胞癌组织及正常胃黏膜组织中8-羟基脱氧鸟苷(8-oxodGsn)水平,探讨DNA氧化损伤与不同类型胃癌临床指标间的关系。方法提取42例胃癌(腺癌28例,印戒细胞癌14例)及癌旁5cm以外的正常组织的DNA,并采用核酸酶P1和碱性磷酸酶消化成单个核苷,再采用高效液相色谱-串联质谱法(LC-MS/MS)检测其8-oxodGsn的水平;同时联合免疫组化法对组织中的8-oxodGs n进行定位分析。结果不同类型胃癌组织与正常组织中8-oxodGsn水平的差异均有统计学意义(均P<0.05)。8-oxodGsn主要分布于肿瘤细胞的细胞质和细胞核内,腺癌细胞中以细胞核分布居多,印戒细胞癌中以细胞质分布居多。不同类型胃癌组织中8-oxodGsn水平在癌组织浸润深度、淋巴结转移程度、远处器官转移程度、临床病理分期等差异均有统计学意义(均P<0.05)。结论 8-oxodGsn水平在两种类型的胃癌组织中均有不同程度的增加,提示DNA氧化损伤可能是胃癌发生、发展的重要因素。
Objective To detect the levels of 8-oxodGsn in gastric adenocarcinoma, signet-ring cell carcinoma and normal gastric mucosa and to explore the relationship between DNA oxidative damage and clinical indexes of different types of gastric cancer. Methods DNA was extracted from 42 cases of gastric cancer (28 cases of adenocarcinoma, 14 cases of signet-ring cell carcinoma) and normal tissues other than 5cm. The DNA was digested with nuclease P1 and alkaline phosphatase into single nucleosides. The level of 8-oxodGsn was detected by LC-MS / MS. The localization of 8-oxodGsn in the tissues was also analyzed by immunohistochemistry. Results The differences of 8-oxodGsn in different types of gastric cancer tissues and normal tissues were statistically significant (all P <0.05). 8-oxodGsn is mainly distributed in the cytoplasm and nucleus of tumor cells. Most of the adenocarcinoma cells are located in the nucleus, while most of the signet ring cell carcinomas are cytoplasm. The levels of 8-oxodGsn in different types of gastric cancer tissues were significantly different (all P <0.05) in the depth of invasion, lymph node metastasis, distant organ metastasis and clinical pathological stage. Conclusion The level of 8-oxodGsn increased in both types of gastric cancer to some extent, suggesting that DNA oxidative damage may be an important factor in the occurrence and development of gastric cancer.