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目的:探讨Kras癌基因在子宫内膜良恶性组织中的表达及与子宫内膜癌发生、发展的关系。方法:采用抗生物蛋白链菌素标记(LSAB)法检测33例子宫内膜癌、31例子宫内膜非典型增生、39例子宫内膜单纯型增生及31例正常子宫内膜标本中Kras癌基因的表达。结果:Kras癌基因在正常子宫内膜、单纯型增生、非典型增生及子宫内膜癌中的阳性表达率分别为35.48%、35.90%、41.94%、69.70%,其阳性表达率随着病变程度的加重而升高(r=0.2853,P<0.05),正常子宫内膜及单纯型增生与非典型增生及子宫内膜癌之间差异有统计学意义(P<0.05)。低分化子宫内膜癌中Kras癌基因阳性表达率明显高于中高分化者,Ⅱ、Ⅲ期子宫内膜癌中Kras癌基因阳性表达率明显高于Ⅰ期(P均<0.05)。Kras癌基因阳性表达率与其年龄及绝经情况无关。结论:Kras癌基因在子宫内膜癌发生、发展中起重要的作用,Kras癌基因的检测对于子宫内膜癌早期诊断、估计预后有一定的意义。
Objective: To investigate the expression of Kras oncogene in benign and malignant endometrium and its relationship with the occurrence and development of endometrial carcinoma. Methods: 33 cases of endometrial carcinoma, 31 cases of endometrial atypical hyperplasia, 39 cases of simple endometrial hyperplasia and 31 cases of normal endometrial carcinoma were detected by LSAB. Gene expression. Results: The positive rates of Kras oncogene in normal endometrium, simple hyperplasia, atypical hyperplasia and endometrial carcinoma were 35.48%, 35.90%, 41.94% and 69.70%, respectively. The positive rate of Kras oncogene was higher with the degree of lesion (R = 0.2853, P <0.05). The difference between normal endometrium, simple hyperplasia and atypical hyperplasia and endometrial carcinoma was statistically significant (P <0.05). The positive rate of Kras oncogene in poorly differentiated endometrial carcinoma was significantly higher than that in moderately well differentiated. The positive rate of Kras oncogene in stage Ⅱ and Ⅲ endometrial carcinoma was significantly higher than that in stage Ⅰ (all P <0.05). The positive rate of Kras oncogene has nothing to do with its age and menopause. Conclusion: Kras oncogene plays an important role in the development and progression of endometrial carcinoma. The detection of Kras oncogene has certain significance for the early diagnosis and prognosis of endometrial carcinoma.