根据百合(Lilium brownii var.viridulum)常见的黄瓜花叶病毒(CMV)、百合斑驳病毒(LMoV)、无症病毒(LSV)引物序列,设计2组特异引物,以18S rRNA为内参基因,采用多重RT-PCR,2组引物均能扩增得到LMoV和LSV预期大小一致的片段,未检测到CMV,检测结果不受病株取材部位的影响。该方法的建立为百合脱毒苗的规模化生产提供了高效检测技术。 Two groups of specific primers were designed according to the common cucumber mosaic virus (CMV), lily mottle virus (LMoV), and disease-free virus (LSV) primer sequences in Lilium brownii var. Viridulum. 18S rRNA was used as an internal reference gene, Both RT-PCR and PCR amplification could amplify the expected fragments of LMoV and LSV. CMV was not detected and the results were not influenced by the site of the infected plants. The establishment of this method provides an efficient detection technique for the mass production of virus-free vaccine Lily.